硫酸头孢喹肟注射液在猪体内的药代动力学及生物等效性研究

为了研究硫酸头孢喹肟注射液在猪体内的药代动力学及生物等效性,将10头健康猪随机分成两组,按2 mg/kg体重肌肉注射两种硫酸头孢喹肟注射液,采用高效液相色谱法测定血浆中硫酸头孢喹肟的浓度,计算主要药代动力学参数,评价两制剂的生物等效性.肌肉注射两种硫酸头孢喹肟注射液后两组的消除半衰期(T1/2β)、达峰浓度(Cmax)、曲线下面积(AUC)等参数差异不显著(P＞0.05).采用双单侧t检验进行判断,受试组AUC 90％置信区间为108.2％ ～ 115.3％,在对照组均值的80％～120％范围内;Cmax90％置信区间为91.9％～115.3％,在对照组均值的70％ ～130％范围内.两种产品在猪体内具有生物等效性和类似的药代动力学,为临床给药方案的制定和临床合理用药提供了参考.     

扑杀病害奶牛时静松灵和敌敌畏联合应用效果观察

布鲁氏菌病（以下简称布病）和牛结核病为人畜共患病,我国将＂两病＂规定为二类动物疫病。尤其是布病对人畜普遍易感,且经接触、空气、黏膜多途径传播、感染,疫情发生后,按照＂检、杀、免、消＂的防治原则,进行科学的果断处置,使疫情得到控制,对提高人口质量,促进畜牧业健康发展具有极为重要的意义。在扑杀过程中,静松灵（Xylazine Hydrochloride Injection）和敌敌畏（DD-VP）的联合应用,成本低,省时省力,符合动物福利原则,虽不是完全的安乐死,但使动物痛苦得到了降低,值得推广。     

白花蛇舌草注射液对荷瘤小鼠免疫功能影响的研究

研究白花蛇舌草注射液对荷瘤小鼠免疫功能的影响。应用比色法,分别测定白花蛇舌草注射液在高(15 mg生药/kg)、中(7.5 mg生药/kg)、低(3.75 mg生药/kg)3种剂量作用下,荷瘤小鼠脾脏NK细胞毒指数、脾细胞的增殖、腹腔巨噬细胞吞噬百分率及指数、血清凝集素活性和脾细胞IL-2活性的变化。与荷瘤对照组比较,白花蛇舌草注射液各剂量组可显著提高NK细胞活性(P0.01,P0.05);增强脾细胞增殖(P0.01,P0.05);显著提高腹腔巨噬细胞的吞噬功能(P0.001,P0.01,P0.05);显著增加血清中抗羊红细胞凝集素值(P0.001、P0.01);高、中剂量组可明显提高脾脏IL-2活性(P0.01,P0.05)。白花蛇舌草注射液具有显著提高荷瘤小鼠免疫功能的作用。     

不同施肥结构对番茄营养品质和硝酸盐含量的影响

研究了不同施肥结构对番茄营养品质和硝酸盐含量的影响。试验结果表明,8个处理中,有机肥 硫酸铵 过磷酸钙 硫酸钾处理的维生素C、可溶性糖含量,糖酸比最高;硝酸盐含量最低,为167mg/kg;硝酸还原酶活性最高。因此,有机肥 硫酸铵 过磷酸钙 硫酸钾处理是番茄栽培的最佳施肥结构。     

促进云南松母树林开花结实研究

云南松(Pinus yunnanensis)木材质量下降,其主要原因是用遗传品质低劣的种子造林。为保证云南松材质优良,提高林分经济价值,必须选用优质种子造林。优质种源基地建立以后,如何使母树早结实、多结实,作者在云南省永仁县白马河林场云南松母树林中进行了试验研究。经过四年的实施、观察、分析得出下列结论:对云南母树施氮肥,种子产量增加265％;施硼砂,种子产量增加175.32％;在树干上注射赤霉素和高锰酸钾,种子产量增加43.76％和87.66％;对云南松母树树冠适当修剪能改变树冠的形状,增加结实层和树冠表面积,这些措施既不会改变云南松的遗传特性,又能提高种子产量,在生产上简单易行,建议在林木种子生产部门试用。     

阿莫西林钠对奶牛乳房炎的防治试验

对奶牛临床型乳房炎的治疗试验结果表明,以5 mg/kg体重,肌肉注射阿莫西林钠,1日2次,连用3 d,治愈率达91.3%,对奶牛隐性乳房炎的治疗结果表明:治疗组产犊后隐性乳房炎乳区阳性率分别为78.6%和7.1%,与青霉素-链霉素粉针药物对照组比较,差异显著(P<0.05)。     

"母犊素"对黄牛性别影响的研究

“母犊素”是一种生殖生理调控的激素类药品。喀什地区2004～2005年在6个县的68个冷配站实施“母犊素”的推广使用,目的在于改善冷配母牛母犊出生率偏低的现象,提高农牧民参与黄牛改良的积极性。项目实施参试牛只达3 500头,随机调查产犊头数1 618头,母犊率平均达到58．90％,比未使用“母犊素”处理的母牛产母犊率42．66％净提高16．24％,相对提高38．07％。试验证明,用“母犊素”处理配种母牛,不仅能大幅度提高母犊出生率,还能提高母牛的部分繁殖性能,值得在黄牛改良中推广应用。     

Live foals produced from sperm-injected oocytes derived from pregnant mares

Recently, in vitro fertilization (IVF) in the horse has met with less than anticipated results. Various problems associated with equine IVF include: (1) the inability to collect large numbers of good quality oocytes, (2) the alteration of the zona pellucida associated with in vitro maturation of equine oocytes, and (3) the improper preparation of equine sperm cells for IVF of these oocytes. Therefore, this study was conducted to achieve fertilization via sperm injection of equine oocytes and to produce live offspring from this IVF procedure. Oocytes were collected by transvaginal ultrasound-guided oocyte retrieval procedures from early pregnant mares of mixed breeds (day 14 to day 70 of pregnancy) and were matured in vitro and subjected to intracytoplasmic sperm injection (ICSI). Injected oocytes were then cultured for 48 hours in either TCM-199 or P-1 medium (glucose and phosphate-free medium) supplemented with 15% fetal bovine serum. Cleavage rates for embryos cultured in the two culture media were different (47% vs. 63% in TCM-199 and P-1, respectively). Also, four Grade 1 embryos were surgically transferred into the oviducts of four recipient mares (one embryo/mare) at 48 hours post-ICSI, with three pregnancies (75%) developing as ultrasonically demonstrated by the presence of an embryonic vesicle in the uterine body by day 16 post-ICSI. On June 23rd one live filly was born after 328 days of gestation and subsequently, a second healthy filly was born after 319 days of gestation. To our knowledge, this is the first report of live foals resulting from in vitro fertilization (via ICSI) of in vitro matured oocytes recovered from pregnant mares using an efficient, repeatable transvaginal ultrasound-guided procedure.